miR-425-5p in the early diagnosis of neonatal brain injury and its involvement in the inflammatory response of brain injury via WTAP.
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OBJECTIVES: Neonatal brain injury (NBI) is one of the common acute and critical illnesses that lead to neonatal death and disability. This article aims to explore miR-425-5p in neonates with different degrees of brain injury and its potential molecular mechanism. METHOD: The researchers used RT-qPCR to measure the level of miR-425-5p in NBI serum and evaluated its diagnostic and predictive capabilities for NBI severity through ROC curve and multivariate logistic regression analysis. In vitro experiments, the effect of miR-425-5p on lipopolysaccharide-induced microglial cell viability was detected using the CCK8 assay, while inflammation and oxidative stress levels were evaluated via ELISA. Finally, the relationship between miR-425-5p and wilms tumor 1-associating protein (WTAP) was verified using dual-luciferase assay and Pearson correlation analysis. RESULTS: miR-425-5p was significantly reduced in the group of neonates with moderate to severe brain injury. miR-425-5p demonstrates excellent diagnostic efficacy for the severity of brain injury (AUC = 0.842) and can serve as a potential protective factor for neonates with brain injury. In LPS-induced microglial cells, miR-425-5p-mimic enhanced cell viability, reduced the release of inflammatory factors, and attenuated oxidative stress levels. Meanwhile, WTAP is a direct target of miR-425-5p, and the two exhibit a negative correlation. CONCLUSIONS: miR-425-5p is lowly expressed in the group of moderate to severe neonatal brain injury and may participate in the inflammatory response of brain injury through WTAP.